{"id":7327,"date":"2022-04-08T13:53:02","date_gmt":"2022-04-08T11:53:02","guid":{"rendered":"https:\/\/services.cbmed.at\/html\/old\/?p=7327"},"modified":"2022-06-28T10:06:38","modified_gmt":"2022-06-28T08:06:38","slug":"in-vitro-experiments","status":"publish","type":"post","link":"https:\/\/services.cbmed.at\/html\/old\/?p=7327","title":{"rendered":"In vitro experiments"},"content":{"rendered":"<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#783986; '  itemprop=\"text\" ><h2><b>In vitro experiments<\/b><\/h2>\n<\/div><\/section>\n<div  class=\"tabcontainer   top_tab   avia-builder-el-1  el_after_av_textblock  el_before_av_section  avia-builder-el-last \">\n\n<section class=\"av_tab_section\"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" >    <div aria-controls=\"tab-id-1\" role=\"tab\" tabindex=\"0\" data-fake-id=\"#tab-id-1\" class=\"tab active_tab\"  itemprop=\"headline\" ><span class='tab_icon' aria-hidden='true' data-av_icon='\ue8bd' data-av_iconfont='entypo-fontello'><\/span>In vitro cultivation of gut microbiomes<\/div>\n    <div id=\"tab-id-1-container\" class=\"tab_content active_tab_content\" aria-hidden=\"false\">\n        <div class=\"tab_inner_content invers-color\"  itemprop=\"text\" >\n<p><a href=\"https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/Slide1-1.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-7350\" src=\"https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/Slide1-1.jpeg\" alt=\"\" width=\"900\" height=\"506\" \/><\/a><\/p>\n\n        <\/div>\n    <\/div>\n<\/section>\n\n<\/div>\n\n<\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_1'  class='avia-section main_color avia-section-default avia-no-border-styling avia-bg-style-scroll  avia-builder-el-2  el_after_av_tab_container  el_before_av_section   container_wrap fullsize' style='background-color: #793282;  '  ><div class='container' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-7327'><div class='entry-content-wrapper clearfix'>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#ffffff; '  itemprop=\"text\" ><h2><b>In vitro cultivation of gut microbiomes<\/b><\/h2>\n<\/div><\/section>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-4  el_after_av_textblock  el_before_av_one_half  column-top-margin\" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><a href=\"https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1.png\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-7328 alignleft\" src=\"https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-1030x793.png\" alt=\"\" width=\"655\" height=\"505\" srcset=\"https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-1030x793.png 1030w, https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-300x231.png 300w, https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-768x591.png 768w, https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-1500x1155.png 1500w, https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/In-vitro1-705x543.png 705w\" sizes=\"auto, (max-width: 655px) 100vw, 655px\" \/><\/a><\/p>\n<\/div><\/section><\/div><div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-6  el_after_av_one_half  avia-builder-el-last  column-top-margin\" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#ffffff; '  itemprop=\"text\" ><ul>\n<li>Stability reached after 48h<\/li>\n<li>&gt;90% of OTUs retained<\/li>\n<li>&gt;90% of genera with &gt;1% abundance retained<\/li>\n<li>stable for 120h (and beyond)<\/li>\n<li>highly reproducible results<\/li>\n<li>Total quality control (LIMS)<\/li>\n<li>Full control of temperature, pH, oxygen, cell growth<\/li>\n<li>Fully anaerobic workflow<\/li>\n<\/ul>\n<\/div><\/section><\/div>\n\n<\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_2'  class='avia-section main_color avia-section-default avia-no-border-styling avia-bg-style-scroll  avia-builder-el-8  el_after_av_section  el_before_av_section   container_wrap fullsize' style=' '  ><div class='container' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-7327'><div class='entry-content-wrapper clearfix'>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#783986; '  itemprop=\"text\" ><h2><b><i>in vitro <\/i><\/b><b>gut barrier model<\/b><\/h2>\n<\/div><\/section>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-10  el_after_av_textblock  el_before_av_one_half  column-top-margin\" style='border-radius:0px; '><p><div  class='avia-image-container  av-styling-    avia-builder-el-11  el_before_av_textblock  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/invitro2.png' alt='' title='invitro2' height=\"368\" width=\"629\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><i>Measurement <\/i><i>of<\/i> <i>transepithelia<\/i> <i>resistance<\/i> <i>with<\/i><i> a Voltohmmeter <\/i><i>and<\/i><i> a Ussing <\/i><i>chamber<\/i> <i>and<\/i> <i>morphological<\/i> <i>assessment<\/i> <i>of<\/i> <i>cell-cell<\/i> <i>connections<\/i><\/p>\n<\/div><\/section><\/p><\/div><div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-13  el_after_av_one_half  avia-builder-el-last  column-top-margin\" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><ul>\n<li>Caco2 based model<\/li>\n<li>TEER (Voltohmmeter and Ussing chamber)<\/li>\n<li>Transwell cultures<\/li>\n<li>variety of readouts: cytokine production in supernatant, cellular gene and protein expression, visualization with immunofluorescence<\/li>\n<li>assessment of \u201dtortuosity\u201d (AI based)<\/li>\n<\/ul>\n<\/div><\/section><\/div>\n\n<\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_3'  class='avia-section main_color avia-section-default avia-no-border-styling avia-bg-style-scroll  avia-builder-el-15  el_after_av_section  avia-builder-el-last   container_wrap fullsize' style='background-color: #793282;  '  ><div class='container' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-7327'><div class='entry-content-wrapper clearfix'>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#ffffff; '  itemprop=\"text\" ><h2><b><i>in vitro <\/i><\/b><b>innate immune model<\/b><\/h2>\n<\/div><\/section>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-17  el_after_av_textblock  el_before_av_one_half  column-top-margin\" style='border-radius:0px; '><p><div  class='avia-image-container  av-styling-    avia-builder-el-18  el_before_av_textblock  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/services.cbmed.at\/html\/old\/wp-content\/uploads\/2022\/04\/invitro3.png' alt='' title='invitro3' height=\"383\" width=\"560\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#ffffff; '  itemprop=\"text\" ><p>Induction of ROS production in human neutrophils in response to probiotic strains alone (left) and\u00a0 pathogens of interest + probiotic strains (right)<\/p>\n<\/div><\/section><\/p><\/div><div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-20  el_after_av_one_half  avia-builder-el-last  column-top-margin\" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  av_inherit_color '  style='color:#ffffff; '  itemprop=\"text\" ><ul>\n<li>Human neutrophils<\/li>\n<li>Large set of readouts<\/li>\n<li>ROS production<\/li>\n<li>phagocytosis<\/li>\n<li>chemotaxis<\/li>\n<li>NET formation<\/li>\n<li>protein expression (e.g. calprotectin)<\/li>\n<\/ul>\n<\/div><\/section><\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":12,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7327","post","type-post","status-publish","format-standard","hentry","category-allgemein"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.13 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>In vitro experiments | CBmed GmbH Center for Biomarker Research in Medicine<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" 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